Accela High Speed LC-Thermo Scientific

Accela* High Speed LC

	Thermo Scientific* Accela High Speed LC provides fast, efficient chromatographic separations over a range of flowrates and pressures for HPLC and UHPLC in a single system using the industry's only quaternary high pressure pump.

Product Detail

	Thermo Scientific chromatography instruments allow users to achieve shorter run times with excellent peak symmetry and superior data quality Accela is designed to optimize performance of sub-two micron particle columns, providing seamless operation from conventional LC pressures up to 1250 bar for U-HPLC separations of complex matrices. Rapid gradient formation with extremely low delay volumes Maximum application flexibility with HPLC/U-HPLC in a single system Precise flow and gradients with proprietary Force Feedback Control (FFC) technology Streamlined integration across a full range of LC & MS detectors Accela 1250 Pump Accelerated method development time with quaternary solvent delivery HPLC & U-HPLC applications up to 1250 bar Extremely fast cycle with a 70µL delay volume FFC technology ensures consistent baseline reproducibility Accela 600 Pump Accelerated method development time with quaternary solvent delivery Greater application flexibility with flow rates up to 5mL/min at 600 bar Minimum gradient equilibration time with a 90µL delay volume FFC technology eliminates the need for pulse dampeners and reduces maintenance costs Accela Open Autosampler Maximum flexibility with unmatched reliability and robustness Customizable temperature controlled sample formats Integrated Dynamic Load and Wash capability resulting in extremely low carryover (0.003% chlorhexidine) Saves precious bench space with a customizable open frame architecture in a stackable design Accela Autosampler Optimized for high-throughput applications and sub two micron column particles Maximum reproducibility with isothermal injection and separation Greater application flexibility with multiple sample formats Superior robustness with a diamond coated high pressure valve Accela Photo Diode Array Detector Highest detection sensitivity with proprietary LightPipe technology Designed for U-HPLC separations with a 1cm path length, 2µL LightPipe flowcell Maximum peak resolution with fiberoptic beam shaper Easy maintenance with integrated wavelength validation Accela Photo Diode Array Detector Highest detection sensitivity with proprietary LightPipe technology Designed for U-HPLC separations with a 1cm path length, 2µL LightPipe flowcell Maximum peak resolution with fiberoptic beam shaper Easy maintenance with integrated wavelength validation Accela UV/Vis Detector Unlimited applications flexibility with externally mounted flow cells Simultaneous detection at two selectable wavelengths in the UV or the visible range of the spectrum Instrument versatility with two analog outputs Simple maintenance with pre-aligned deuterium and tungsten lamps Accela Refractive Index (RI) Detector High sensitivity HPLC detection Faster throughput with a low delay volume flowcell Automatic lamp brightness compensation for flowcell stains Optimum sensitivity and selectivity with Thermo Scientific columns Ideal for: Forensics, Pharmaceutical, Biodiesel Analysis, Food Safety, Environmental, Forensics, Evidence Analysis, Drug Screening, Metabolite Analysis

Accela* High Speed LC

Product#	Price	Description

6005760030	-	Performs HPLC and UHPLC, upper pressure limit 15,000 psi	Select

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Successful HPLC Operation: A Troubleshooting Guide (CD)

FREE 2008/2009 Chromatography Columns & Consumables Catalog

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LCQ Fleet Brochure (PDF)

U-HPLC in the Analysis of Phenolic Pollutants in Water (PDF)

Method Transfer from HPLC to UHPLC for Increased Speed and Resolution (PDF)

Accela Autosampler Product Specification (PDF)

Accela PDA Detector Product Specification (PDF)

Accela High Speed Pump Product Specification (PDF)

Accela High Speed LC System Brochure (PDF)

Thermo Scientific Sistemas LC Accela

Specifications:

Absorbance Linearity :

<5% up to 2.0 AU at 254nm

Absorbance Non-Linearity :

<5% at 2.0 AU at 257nm

Absorbance Range :

0.0005 AUFS to + 3.0 AUFS

Ambient Environment :

4 to 40°C, 5 to 80% relative humidity, (non condensing)

Analog Outputs :

20-bit digital/analog conversion, unattenuated at 10mV/AU,100mV/AU, or 1.0V/AU

Auto Zero Range :

±10 x 10-⁵ RIU

Auto Zero Resolution :

±10 x 10-⁹ RIU

Cell Dimensions :

10mm, 15 µL standard analytical
50mm, 10 µL LightPipe
10mm, 2 µL LightPipe

Cell Pressure Range :

0 to 1000psi

Column Oven :

5 to 95°C ± 0.5°C selectable in 1°C increments

Communications :

Remote Inputs
Pump Ready and Inject Hold

Compositional Accuracy :

±0.50%

Compositional Precision :

<0.2% RSD

Degasser Hold Volume :

<500µL per channel

Degasser Type :

Integral, self-adjusting, 4 channel vacuum membrane (PTFE AF)

Delay Volume :

70µL

Dimensions :

15.74D x 10.23W x 6.29 in. H (40 x 26 x 16cm)

Diode Spacing :

1.2nm

Diodes :

512

Discrete Channels :

Two wavelength-selectable channels

Drift :

<1 mAU/hr at 254nm and 546nm using a 50mm LightPipe flow cell
<0.1 mAU/hr at 254nm and 546nm using a 10mm standard analytical quartz flow cell

Drift/Unit Temperature :

2 x 10-⁷ RIU/°C

Drift/Unit Time :

2 x 10-⁷ RIU/h

Flow Rate Accuracy :

±0.5% at set point or +1µL/min whichever is greater

Flow Rate Resolution :

0.1µL/min

Flowcell Pressure :

5kg/cm² max

Flowcell Volume :

8µL

Injection Volume :

25µL loop standard, up to 1000µL injections with larger loops and syringe

Integrator Output :

0 to 1V (512 x 10-⁵ RIU/FS)

Light Source :

Pre-aligned Deuterium and Tungsten lamps

Linearity :

<60 x 10-⁵ RIU

Maximum Flow Rate :

10mL/min

Maximum Operating Pressure :

18,000psi

Measuring Method :

Deflection method

Minimum Flow Rate :

1.0µL/min (isocratic)

Minimum Sample Volume :

1µL can be injected from a 5µL sample

Needle Height :

Programmable in 0.1mm increments. Active vial bottom search selectable on/off

Pressure Range :

0 to 1250 bar (0 to 18,130psi)

Pressure Resolution :

0.01 bar (0.15psi)

Primary Piston Volume :

23µL

Pulse Dampener :

No pulse dampener

Recommended Flow Rate :

0.2 to 3.0mL/min

Recorder Output :

0 to 10mV

Refractive Index Range :

1.00 to 1.75 RIU

Remote Outputs :

Pump Stop, Autosampler Ready, Inject, and Gradient Start
4 Timed Events

Reproducibility :

<1% RSD (partial loop)

Residual Pulsation :

<0.5 bar amp. (typical)

Response :

FAST/STD/SLOW (3 steps)

Rise Time :

User selectable: 0, 0.1, 0.2, 0.5, 1, 2, 3, 4, or 5 sec

Sample Carryover :

<0.003% chlorhexidine (with two wash solutions) and UV detection (257nm)

Sample Preparation :

Capacity for four 15mL reagent vials for flushing, washing, sample dilution, and/or reagents for sample derivatization

Scan Rate :

User selectable: 4, 5, 6.67, 10 or 20Hz

Short-Term Noise :

<6µAU/cm at 254nm, 2 sec rise time, single wavelength mode using a 50mm LightPipe flow cell
<20µAU/cm at 254nm, 2 sec rise time, single wavelength mode using a 10mm standard analytical quartz flow cell

Short-Term Noise (50 mm flow cell) :

<6µAU/cm at 254nm at 5Hz (20Hz scan rate)
<1.5µAU/cm at 254nm at 5Hz (40Hz scan rate); typical
<0.3µAU/cm at 254nm at 5Hz (80Hz scan rate); typical

Short-term Noise :

2.5 x 10-⁹ RIU

Solvent Capacity :

Quaternary valve system

Spectral Bandwidth :

6nm

Syringe Size :

100µL
Optional syringe sizes available (10 to 5000µL)

Tray Temperature Control :

4 to 40°C

Typical Clean Cycle Time :

Less than 1 minute for Fast Cycle

Variable Volume :

Injection Precision: 1.0% RSD at 5µL and higher

Vial Capacity :

324 vials (2.0mL)
Twelve 96- or 384-well plates
Six 96- or 384-deep well plates

Warm-up Time :

90 min. required to meet noise and drift specifications

Wavelength Accuracy :

±1nm at 254nm and 656nm

Wavelength Calibration :

Using Holmium oxide solution

Wavelength Precision :

<0.01nm

Wavelength Range :

190 to 800nm
Deuterium Lamp: 190 to 365nm
Tungsten Lamp: 366 to 800nm

Wavelength Resolution :

1.2nm (512 pixel array)

Weight :

39.6 lb. (18kg)